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Bone tissue engineering using bone marrow stromal cells and an injectable sodium alginate/gelatin scaffold
Author(s) -
Xia Yang,
Mei Fang,
Duan Yongli,
Gao Ying,
Xiong Zhuo,
Zhang Ting,
Zhang Hongquan
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33232
Subject(s) - scaffold , gelatin , materials science , biomedical engineering , biocompatibility , stromal cell , tissue engineering , bone healing , bone tissue , bone marrow , bone cell , biomaterial , chemistry , pathology , microbiology and biotechnology , anatomy , medicine , biology , biochemistry , metallurgy
Abstract To investigate the potential application of bone marrow stromal cells (BMSCs) and an injectable sodium alginate/gelatin scaffold for bone tissue engineering (BTE). The phenotype of osteogenic BMSCs was examined by mineralized nodules formation and type I collagen expression. Cell proliferation was evaluated by MTT assay. The biocompatibility of scaffold and osteogenic cells were examined by hematoxylin and eosin (H&E) staining. Ectopic bone formation as well as closure of rabbit calvarial critical‐sized defects following scaffold‐cell implantation were analyzed by histological examination and computed tomography (CT) scanning. Spindle‐shaped osteogenic cells of high purity were derived from BMSCs. The osteogenic cells and sodium alginate/gelatin (2:3) scaffold presented fine biocompatibility following cross‐linking with 0.6% of CaCl 2 . After implantation, the scaffold‐cell construct promoted both ectopic bone formation and bone healing in the rabbit calvarial critical‐sized defect model. Our data demonstrated that the sodium alginate/gelatin scaffold could be a suitable biomaterial for bone engineering, and the scaffold‐osteogenic cells construct is a promising alternative approach for the bone healing process. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.