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In vitro evaluation of glass–glass ceramic thermoseed‐induced hyperthermia on human osteosarcoma cell line
Author(s) -
Alcaide María,
RamírezSantillán Cecilia,
Feito María José,
de la Concepción Matesanz María,
RuizHernández Eduardo,
Arcos Daniel,
ValletRegí María,
Portolés María Teresa
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33229
Subject(s) - materials science , osteosarcoma , hyperthermia , in vitro , cell culture , biomedical engineering , bioactive glass , ceramic , glass ceramic , cancer research , composite material , medicine , biology , biochemistry , genetics
The use of biomaterials as implantable thermoseeds under the action of an external magnetic field is a very interesting methodology to focus the heat into the target tumors as osteosarcoma. In this study, biocompatible and bioactive G15GC85 thermoseeds, tailored through the combination of sol–gel glasses (G) with a magnetic glass ceramic (GC), were used to induce hyperthermia on cultured human osteosarcoma cells after exposition to alternating magnetic field (MF, 100 kHz/200 Oe). G15GC85 magnetic glass–glass ceramic thermoseeds induced in vitro effective hyperthermia with drastic reduction in proliferation of human osteosarcoma Saos‐2 cells and high increase of apoptotic cells after two 40 min consecutive sessions of MF. Deep cell morphology alterations were observed after this hyperthermic treatment, and the proteomic analysis revealed modification of gamma actin molecular properties related to cytoskeleton alterations. These results indicate that G15GC85 thermoseeds allow to induce in vitro effective hyperthermia on human osteosarcoma cells. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.