Premium
Peripheral nerve repair of transplanted undifferentiated adipose tissue‐derived stem cells in a biodegradable reinforced nerve conduit
Author(s) -
Shen ChiungChyi,
Yang YiChin,
Liu BaiShuan
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33227
Subject(s) - sciatic nerve , nerve guidance conduit , genipin , regeneration (biology) , adipose tissue , materials science , gelatin , stem cell , tissue engineering , biomedical engineering , peripheral nerve , anatomy , chemistry , medicine , microbiology and biotechnology , biology , chitosan , biochemistry
Abstract This study proposes a biodegradable nerve conduit containing genipin‐cross‐linked gelatin annexed with tricalcium phosphate ceramic particles (genipin‐gelatin‐tricalcium phosphate, GGT) in peripheral nerve regeneration. Firstly, cytotoxicity tests revealed that the GGT‐extracts were not toxic, and promoted the proliferation and neuronal differentiation of adipose tissue‐derived stem cells (ADSCs). Secondly, the GGT composite film effectively supported ADSCs attachment and growth. Additionally, the GGT substrate was biocompatible with the neonatal rat sciatic nerve and produced a beneficial effect on peripheral nerve repair through in vitro tissue culture. Finally, the experiments in this study confirmed the effectiveness of a GGT/ADSCs nerve conduit as a guidance channel for repairing a 10‐mm gap in a rat sciatic nerve. Eight weeks after implantation, the mean recovery index of compound muscle action potentials (CMAPs) was significantly different between the GGT/ADSCs and autografts groups ( p < 0.05), both of which were significantly superior to the GGT group ( p < 0.05). Furthermore, walking track analysis also showed a significantly higher sciatic function index (SFI) score ( p < 0.05) and better toe spreading development in the GGT/ADSCs group than in the autograft group. Histological observations and immunohistochemistry revealed that the morphology and distribution patterns of nerve fibers in the GGT/ADSCs nerve conduits were similar to those of the autografts. The GGT nerve conduit offers a better scaffold for the incorporation of seeding undifferentiated ADSCs, and opens a new avenue to replace autologous nerve grafts for the rapid regeneration of damaged peripheral nerve tissues and an improved approach to patient care. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.