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Protein conformation changes on block copolymer surfaces detected by antibody‐functionalized atomic force microscope tips
Author(s) -
Palacio Manuel L. B.,
Schricker Scott R.,
Bhushan Bharat
Publication year - 2012
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33219
Subject(s) - materials science , copolymer , atomic force microscopy , nanotechnology , crystallography , composite material , polymer , chemistry
Abstract Conformational changes of fibronectin (Fn) deposited on poly(methyl methacrylate) and poly(acrylic acid) block copolymers with identical chemical compositions were detected using an antibody‐functionalized atomic force microscope (AFM) tip. Based on the antibody‐protein adhesive force maps and phase imaging, it was found that the nanomorphology of the triblock copolymer is conducive to the exposure of the arginine‐glycine‐aspartic acid (RGD) groups in Fn. For the first time, X‐ray photoelectron spectroscopy was used to elucidate surface chemical composition and confirm AFM results. The findings demonstrate that block copolymer nanomorphology can be used to regulate protein conformation and potentially cellular response. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.