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Optimized conditions for mesenchymal stem cells to differentiate into osteoblasts on a collagen/hydroxyapatite matrix
Author(s) -
Prosecká E.,
Rampichová M.,
Vojtová L.,
Tvrdík D.,
Melčáková Š.,
Juhasová J.,
Plencner M.,
Jakubová R.,
Jančář J.,
Nečas A.,
Kochová P.,
Klepáček J.,
Tonar Z.,
Amler E.
Publication year - 2011
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33189
Subject(s) - mesenchymal stem cell , scaffold , materials science , biomedical engineering , matrix (chemical analysis) , extracellular matrix , type i collagen , osteoblast , in vitro , microbiology and biotechnology , composite material , chemistry , biology , pathology , biochemistry , medicine
Collagen/hydroxyapatite (HA) composite scaffolds are known to be suitable scaffolds for seeding with mesenchymal stem cells (MSCs) differentiated into osteoblasts and for the in vitro production of artificial bones. However, the optimal collagen/HA ratio remains unclear. Our study confirmed that a higher collagen content increased scaffold stiffness but that a greater stiffness was not sufficient for bone tissue formation, a complex process evidently also dependent on scaffold porosity. We found that the scaffold pore diameter was dependent on the concentration of collagen and HA and that it could play a key role in cell seeding. In conclusion, the optimal scaffold for new bone formation and cell proliferation was found to be a composite scaffold formed from 50 wt % HA in 0.5 wt % collagen I solution. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2011.