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Effects of TiO 2 ‐containing phosphate glasses on solubility and in vitro biocompatibility
Author(s) -
Novajra G.,
VitaleBrovarone C.,
Knowles J. C.,
Maina G.,
Aina V.,
Ghigo D.,
Bergandi L.
Publication year - 2011
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33186
Subject(s) - biocompatibility , dissolution , solubility , materials science , citric acid , phosphate , nuclear chemistry , titanium , titanium dioxide , osteoblast , chemical engineering , in vitro , metallurgy , organic chemistry , chemistry , biochemistry , engineering
Phosphate‐based glasses with different amounts of titanium dioxide (TiO 2 ), having the following molar composition 50P 2 O 5 ‐30CaO‐9Na 2 O‐3SiO 2 ‐3MgO‐(5‐ x )K 2 O‐ x TiO 2 , (where x = 0, 2.5, 5 mol %), were synthesised and characterized in terms of solubility (according to ISO 10993‐14), and in vitro biocompatibility using human MG‐63 osteoblast cells. Dissolution tests were carried out in Tris‐HCl (pH 7.4) to simulate the physiological pH and in citric acid (pH 3.0) to simulate an acidic environment. The weight loss decreased with increasing TiO 2 content, a process further enhanced in acidic medium. TiO 2 reduced the pH changes usually caused by the dissolution products released. Cellular tests showed that all the glasses studied (0–5 mol % TiO 2 ) and TiCl 4 , used to investigate the biocompatibility of titanium ions, did not produce cytotoxic effects on human MG‐63 osteoblasts for up to 5 days in culture. On the basis of these results, we suggest that TiO 2 ‐containing phosphate glasses could be promising substrates for bone tissue engineering applications. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2011.