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Effects of laminin‐coated carbon nanotube/chitosan fibers on guided neurite growth
Author(s) -
Huang YiCheng,
Hsu SungHao,
Kuo WenChun,
ChangChien ChengLun,
Cheng Henrich,
Huang YiYou
Publication year - 2011
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33164
Subject(s) - chitosan , materials science , carbon nanotube , fiber , tissue engineering , composite material , ultimate tensile strength , neural tissue engineering , neurite , nanofiber , biomedical engineering , chemical engineering , in vitro , chemistry , medicine , biochemistry , engineering
Abstract This study assesses the ability and potential of carbon nanotube (CNT)/chitosan to guide axon re‐growth after nerve injuries. The CNT/chitosan fibers were produced via the coagulation and hydrodynamic focusing method. Fiber width and morphology were adjusted using such parameters as syringe pumping rate and the coagulant used. The CNT/chitosan fiber diameters were 50–300 μm for syringe pumping rates of 6–48 mL/h. Polyethylene glycol/NaOH (25%, w/w) solution was a suitable coagulant for forming fibers with small diameters. Physical property tests demonstrate that the CNT/chitosan composites had superior tensile strength and electrical conductivity compared with those of chitosan alone. The MTT and LDH tests reveal that CNT/chitosan composites were not cytotoxic. To improve the neural cell affinity of CNT/chitosan fibers, laminin was incorporated onto fiber surfaces via the oxygen plasma technique; cell adhesion ratio increased significantly from 3.5% to 72.2% with this surface modification. Immunofluorescence staining and SEM imaging indicate that PC12 cells adhered successfully and grew on the laminin (LN)‐coated CNT/chitosan films and fibers. Experimental results show that PC12 grown on LN‐coated CNT/chitosan fibers in vitro extend longitudinally oriented neurites in a manner similar to that of native peripheral nerves. With the inherent electrical properties of CNTs, oriented CNT/chitosan fibers have a potential for use as nerve conduits in nerve tissue engineering. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 99A: 86–93, 2011.