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Polymerizable superoxide dismutase mimetic protects cells encapsulated in poly(ethylene glycol) hydrogels from reactive oxygen species‐mediated damage
Author(s) -
Hume Patrick S.,
Anseth Kristi S.
Publication year - 2011
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33160
Subject(s) - self healing hydrogels , superoxide , ethylene glycol , reactive oxygen species , peg ratio , superoxide dismutase , biophysics , materials science , chemistry , biochemistry , polymer chemistry , oxidative stress , enzyme , organic chemistry , biology , finance , economics
A polymerizable superoxide dismutase mimetic (SODm) was incorporated into poly(ethylene glycol) (PEG) hydrogels to protect encapsulated cells from superoxide‐mediated damage. Superoxide and other small reactive oxygen species (ROS) can cause oxidative damage to donor tissue encapsulated within size exclusion barrier materials. To enzymatically breakdown ROS within biomaterial cell encapsulation systems, Mn(III) Tetrakis[1‐(3‐acryloxy‐propyl)‐4‐pyridyl] porphyrin (MnTTPyP‐acryl), a polymerizable manganese metalloporphyrin SOD mimetic, was photopolymerized with PEG diacrylate (PEGDA) to create functional gels. In unmodified PEG hydrogels, a significant reduction in metabolic activity was observed when encapsulated Min6 β‐cells were challenged with chemically generated superoxide. Cells encapsulated within MnTPPyP‐co‐PEG hydrogels, however, demonstrated greatly improved metabolic activity following various superoxide challenges. Further, cells were encapsulated and cultured for 10 days within MnTPPyP‐co‐PEG hydrogels and challenged with superoxide on days 4, 6, and 8. At the conclusion of this study, cells in blank PEG hydrogels had no observable metabolic activity but when encapsulated in MnTPPyP‐functionalized hydrogels, cells retained 60 ± 5% of the metabolic activity compared to untreated controls. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 99A: 29–37, 2011.