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Photochemical cross‐linking of plastically compressed collagen gel produces an optimal scaffold for corneal tissue engineering
Author(s) -
Mi Shengli,
Khutoryanskiy Vitaliy V.,
Jones Roanne Razalia,
Zhu Xiuping,
Hamley Ian William,
Con Che John
Publication year - 2011
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33152
Subject(s) - biocompatibility , materials science , biomaterial , scaffold , biomedical engineering , tissue engineering , biophysics , cornea , nanotechnology , ophthalmology , medicine , metallurgy , biology
The experiments were designed to use photochemically cross‐linked plastically compressed collagen (PCPCC) gel to support corneal epithelial cells. A plastically compressed collagen (PCC) scaffold was photo cross‐linked by UVA in the presence of riboflavin to form a biomaterial with optimal mechanical properties. The breaking force, rheology, surgical suture strength, transparency, ultrastructure, and cell‐based biocompatibility were compared between PCPCC and PCC gels. The breaking force increased proportionally with an increased concentration of riboflavin. The stress required to reach breaking point of the PCPCC scaffolds was over two times higher compared to the stress necessary to break PCC scaffolds in the presence of 0.1% riboflavin. Rheology results indicated that the structural properties of PCC remain unaltered after UVA cross‐linking. The PCC gels were more easily broken than PCPCC gels when sutured on to bovine corneas. The optical density values of PCPCC and PCC showed no significant differences ( p > 0.05). SEM analyses showed that the collagen fibres within the PCPCC gels were similar in morphology to PCC gels. No difference in cell‐based biocompatibility was seen between the PCPCC and PCC scaffolds in terms of their ability to support the ex vivo expansion of corneal epithelial cells or their subsequent differentiation evidenced by similar levels of cytokeratin 14. In conclusion, PCPCC scaffold is an optimal biomaterial for use in therapeutic tissue engineering of the cornea. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 99A: 1–8, 2011.