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Poly(dimethyl siloxane) surface modification with biosurfactants isolated from probiotic strains
Author(s) -
Pinto S.,
Alves P.,
Santos A. C.,
Matos C. M.,
Oliveiros B.,
Gonçalves S.,
Gudiña E.,
Rodrigues L. R.,
Teixeira J. A.,
Gil M. H.
Publication year - 2011
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33146
Subject(s) - contact angle , materials science , surface modification , x ray photoelectron spectroscopy , attenuated total reflection , biocompatibility , fourier transform infrared spectroscopy , siloxane , chemical engineering , biomaterial , simulated body fluid , contact lens , nanotechnology , polymer , composite material , scanning electron microscope , engineering , metallurgy , physics , optics
Depending on the final application envisaged for a given biomaterial, many surfaces must be modified before use. The material performance in a biological environment is mainly mediated by its surface properties that can be improved using suitable modification methods. The aim of this work was to coat poly(dimethyl siloxane) (PDMS) surfaces with biosurfactants (BSs) and to evaluate how these compounds affect the PDMS surface properties. BSs isolated from four probiotic strains ( Lactococcus lactis , Lactobacillus paracasei , Streptococcus thermophilus A, and Streptococcus thermophilus B) were used. Bare PDMS and PDMS coated with BSs were characterized by contact angle measurements, infrared spectroscopy (ATR‐FTIR), X‐ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM). The influence of the surface modifications on the materials blood compatibility was studied through thrombosis and hemolysis assays. The cytotoxicity of these materials was tested against rat peritoneal macrophages. AFM results demonstrated the successful coating of the surfaces. Also, by contact angle measurements, an increase of the coated surfaces hydrophilicity was seen. Furthermore, XPS analysis indicated a decrease of the silicon content at the surface, and ATR‐FTIR results showed the presence of BS characteristic groups as a consequence of the modification. All the studied materials revealed no toxicity and were found to be nonhemolytic. The proposed approach for the modification of PDMS surfaces was found to be effective and opens new possibilities for the application of these surfaces in the biomedical field. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.