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Endotoxin contamination delays the foreign body reaction
Author(s) -
van Putten Sander M.,
Wübben Maike,
Plantinga Josée A.,
Hennink Wim E.,
van Luyn Marja J. A.,
Harmsen Martin C.
Publication year - 2011
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33144
Subject(s) - in vivo , contamination , biomaterial , macrophage , foreign body giant cell , in vitro , materials science , proinflammatory cytokine , microbiology and biotechnology , immune system , immunology , biology , inflammation , medicine , biochemistry , pathology , nanotechnology , ecology
Biomaterials are at continuous risk of bacterial contamination during production and application. In vivo , bacterial contamination of biomaterials delays the foreign body reaction (FBR). Endotoxins such as lipopolysaccharides (LPS), major constituents of the bacterial cell wall, are potent stimulators of the immune system in vitro and in vivo . In vitro , biomaterials contaminated with LPS induce the production of proinflammatory cytokines by adherent macrophages. This suggests that the presence of endotoxins on biomaterials will intensify the FBR. The effects of LPS on the course of the FBR have never been studied in vivo . In this study, the influence of LPS contamination on the FBR to subcutaneously implanted Puramatrix‐loaded hexamethylenediisocyanate‐crosslinked dermal sheep collagen (HDSC) disks was studied in rats. During the onset phase of the FBR, a massive influx of granulocytes was detected in LPS‐contaminated disks, while their presence was prolonged. IL‐10 production inside LPS‐contaminated disks was increased at days 10 and 21. Macrophage densities were not affected by the presence of LPS. However, macrophage functionality was altered: giant cell formation and biomaterial degradation were delayed by LPS‐contamination up to 21 days. On the basis of these results, we conclude that LPS delays the FBR. This finding indicates that endotoxin contamination has significant implications for the in vivo function of biomaterials and medical devices and emphasizes the importance of endotoxin testing. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.