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Degradation, cytotoxicity, and biocompatibility of NIPAAm‐based thermosensitive, injectable, and bioresorbable polymer hydrogels
Author(s) -
Cui Zhanwu,
Lee Bae Hoon,
Pauken Christine,
Ver Brent L.
Publication year - 2011
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33093
Subject(s) - lower critical solution temperature , materials science , gel permeation chromatography , polymer , copolymer , self healing hydrogels , polymer chemistry , dispersity , biocompatibility , differential scanning calorimetry , acrylate , acrylic acid , chemical engineering , nuclear chemistry , composite material , chemistry , thermodynamics , physics , engineering , metallurgy
Abstract A thermosensitive, injectable, and bioresorbable polymer hydrogel, poly(N‐isopropylacrylamide‐co‐dimethyl‐γ‐butyrolactone acrylate‐co‐acrylic acid) [poly(NDBA)], was synthesized by radical copolymerization with 7.00 mol % dimethyl‐γ‐butyrolactone acrylate in tetrahydrofuran. The chemical composition was determined by acid titration in conjunction with 1 H NMR quantification. The molecular weight and polydispersity were determined by gel permeation chromatography in conjunction with static light scattering. The degradation properties of the polymer hydrogel were characterized using differential scanning calorimetry, percentage mass loss, cloud point test, and swelling ratio over time. It was found that the initial lower critical solution temperature (LCST) of the polymer is between room temperature and body temperature and that it takes about 2 weeks for the LCST to surpass body temperature under physiological conditions. An indirect cytotoxicity test indicated that this copolymer has relatively low cytotoxicity as seen with 3T3 fibroblast cells. The in vivo ‐gelation and degradation study showed good agreement with in vitro ‐degradation findings, and no detrimental effects to adjacent tissues were observed after the complete dissolution of the polymer. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.