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CaSiO 3 microstructure modulating the in vitro and in vivo bioactivity of poly(lactide‐ co ‐glycolide) microspheres
Author(s) -
Wu Chengtie,
Zhang Yufeng,
Fan Wei,
Ke Xuebin,
Hu Xuye,
Zhou Yinghong,
Xiao Yin
Publication year - 2011
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33092
Subject(s) - materials science , plga , biocompatibility , in vivo , simulated body fluid , biomedical engineering , microstructure , lactide , bone tissue , apatite , bone healing , chemical engineering , scanning electron microscope , nanotechnology , polymer , composite material , surgery , medicine , copolymer , nanoparticle , metallurgy , microbiology and biotechnology , engineering , biology
Abstract Poly(lactide‐ co ‐glycolide) (PLGA) microspheres have been used for regenerative medicine due to their ability for drug delivery and generally good biocompatibility, but they lack adequate bioactivity for bone repair application. CaSiO 3 (CS) has been proposed as a new class of material suitable for bone tissue repair due to its excellent bioactivity. In this study, we set out to incorporate CS into PLGA microspheres to investigate how the phase structure (amorphous and crystal) of CS influences the in vitro and in vivo bioactivity of the composite microspheres, with a view to the application for bone regeneration. X‐ray diffraction (XRD), N 2 adsorption‐desorption analysis, and scanning electron microscopy (SEM) were used to analyze the phase structure, surface area/pore volume, and microstructure of amorphous CS (aCS) and crystal CS (cCS), as well as their composite microspheres. The in vitro bioactivity of aCS and cCS–PLGA microspheres was evaluated by investigating their apatite‐mineralization ability in simulated body fluids (SBF) and the viability of human bone mesenchymal stem cells (BMSCs). The in vivo bioactivity was investigated by measuring their de novo bone‐formation ability. The results showed that the incorporation of both aCS and cCS enhanced the in vitro and in vivo bioactivity of PLGA microspheres. cCS/PLGA microspheres improved better in vitro BMSC viability and de novo bone‐formation ability in vivo , compared to aCS/PLGA microspheres. Our study indicates that controlling the phase structure of CS is a promising method to modulate the bioactivity of polymer microsphere system for potential bone tissue regeneration. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.