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The tissue response to photopolymerized PEG‐p(HPMAm‐lactate)‐based hydrogels
Author(s) -
Censi Roberta,
van Putten Sander,
Vermonden Tina,
di Martino Piera,
van Nostrum Cornelus F.,
Harmsen Martin C.,
Bank Ruud A.,
Hennink Wim E.
Publication year - 2011
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33048
Subject(s) - self healing hydrogels , in vivo , materials science , biomaterial , biocompatibility , tissue engineering , methacrylamide , infiltration (hvac) , biomedical engineering , polymer , biophysics , polymer chemistry , copolymer , nanotechnology , medicine , composite material , acrylamide , biology , microbiology and biotechnology , metallurgy
Hydrogels are three‐dimensional networks of crosslinked hydrophilic polymers widely used for protein delivery and tissue engineering. To be eligible for in vivo applications, the hydrogels should not evoke an adverse tissue response. In this study the angiogenic and inflammatory responses in vivo after implantation of photopolymerized thermosensitive poly(hydroxypropyl methacrylamide lactate)‐poly(ethyl copolymer hydrogels are investigated. Hydrogels consisting of polymers with different crosslink densities were subcutaneously implanted in Balb/c mice and histological evaluation of the tissue response was performed. The implants showed an acute and localized inflammatory reaction upon implantation, mainly characterized by a strong infiltration of granulocytes. The acute inflammatory reaction was followed by a milder chronic inflammation which was characterized by infiltration of macrophages and persistent but decreasing levels of granulocytes. The number of macrophages and blood vessels was associated with the biodegradation and resorption of the biomaterial and increased in time as the degradation of the materials progressed. The observed degradation rates in vivo correlated well with previously observed in vitro degradation rates, which suggests that hydrolysis is the main mechanism governing the degradation. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.