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Simultaneous biochemical and topographical patterning on curved surfaces using biocompatible sacrificial molds
Author(s) -
Fernandez Javier G.,
Samitier Josep,
Mills Christopher A.
Publication year - 2011
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33038
Subject(s) - streptavidin , materials science , lithography , biocompatible material , polymer , nanotechnology , soft lithography , mold , fabrication , composite material , biotin , biomedical engineering , optoelectronics , chemistry , medicine , biochemistry , alternative medicine , pathology
A method for the simultaneous (bio)chemical and topographical patterning of enclosed structures in poly(dimethyl siloxane) (PDMS) is presented. The simultaneous chemical and topography transference uses a water‐soluble chitosan sacrificial mold to impart a predefined pattern with micrometric accuracy to a PDMS replica. The method is compared to conventional soft‐lithography techniques on planar surfaces. Its functionality is demonstrated by the transference of streptavidin directly to the surface of the three‐dimensional PDMS structures as well as indirectly using streptavidin‐loaded latex nanoparticles. The streptavidin immobilized on the PDMS is tested for bioactivity by coupling with fluorescently labeled biotin. This proves that the streptavidin is immobilized on the PDMS surface, not in the bulk of the polymer, and is therefore accessible for use as signaling/binding element in micro and bioengineering. The use of a biocompatible polymer and processes enables the technique to be used for the chemical patterning of tissue constructions. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.