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Surface immobilization of elastin‐like polypeptides using fluorinated surface modifying additives
Author(s) -
Blit Patrick H.,
Battiston Kyle G.,
Woodhouse Kimberly A.,
Santerre J. Paul
Publication year - 2011
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33022
Subject(s) - materials science , surface modification , x ray photoelectron spectroscopy , peptide , elastin , adhesion , amide , polycarbonate , biophysics , cell adhesion , contact angle , chemical engineering , chemistry , biochemistry , composite material , medicine , pathology , engineering , biology
Elastin‐like polypeptide (ELP) surface modification represents a valuable approach for the development of biomaterials in a wide range of medical applications. In this study, ELP surface modification has been achieved through the use of elastin cross‐linking peptide (ECP) bioactive fluorinated surface modifiers (ECP‐BFSMs). The synthesis of low molecular weight fluorinated additives was described and their subsequent blending with a base polycarbonate urethane (PCNU) was shown to successfully enrich the surface to allow for ELP surface cross‐linking via lysine moieties on the peptide segments of the ECP‐BFSMs. The kinetics for the surface migration of fluorescent ECP‐BFSMs was studied over a 2‐week period by two‐photon confocal microscopy. A decrease in advancing contact angle from 87.9° to 75.3° was observed for ECP‐BFSM modified PCNU and was associated with the presence of ECP peptides on the surface. X‐ray photoelectron spectroscopy demonstrated an increase in surface atomic percent of fluorine (from 0.2 to 7.2%) and nitrogen (from 1.0 to 3.0%) associated with the surface localization of fluoro groups and amide groups associated with the peptides in the ECP‐BFSMs. A further increase in surface atomic percent of nitrogen (from 3.0 to 8.3%) was observed after ELP surface cross‐linking. These ELP‐modified surfaces were shown to promote increased smooth muscle cell adhesion, spreading and retention over a 7‐day culture period relative to their non‐ELP4 analogs. This novel surface modifying additive approach may be used for various biomimetic applications since it generates a stable ECM‐like surface retained onto a relatively inert fluorinated background. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.