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Influence of polysaccharide composition on the biocompatibility of pullulan/dextran‐based hydrogels
Author(s) -
Abed Aicha,
Assoul Nabila,
Ba Maguette,
Derkaoui Sidi Mohamed,
Portes Patrick,
Louedec Liliane,
Flaud Patrice,
Bataille Isabelle,
Letourneur Didier,
MeddahiPellé Anne
Publication year - 2011
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.33007
Subject(s) - pullulan , self healing hydrogels , dextran , biocompatibility , materials science , biomaterial , tissue engineering , in vivo , biomedical engineering , extracellular matrix , polymer , polysaccharide , chemical engineering , polymer chemistry , chemistry , composite material , chromatography , nanotechnology , biochemistry , medicine , microbiology and biotechnology , biology , engineering , metallurgy
Abstract The implantation of a biomaterial for tissue engineering requires the presence of a suitable scaffold on which the tissue repair and regeneration will take place. Polymers have been frequently used for that purpose because they show similar properties to that of the natural extracellular matrix. Scaffold properties and biocompatibility are modulated by the composition of the polymers used. In this work four polysaccharide‐based hydrogels (PSH) made of dextran and pullulan were synthesized. Their in vitro properties were determined and then tested in vivo in a rat model. As pullulan concentration increased in dextran hydrogels, the glass transition temperature and the maximum modulus decreased. In vitro degradation studies for 30 days demonstrated no significant degradation of PSH except for 100% pullulan hydrogel. In vivo tissue response evaluated 30 days after PSH subcutaneous implantation in rats indicated that all PSH were surrounded by a fibrous capsule. Adding pullulan to dextran induced an increased inflammatory reaction compared to PSH‐D (100% dextran) or PSH‐D 75 P 25 (75% dextran) . This in vitro and in vivo data can be used in the design of hydrogels appropriate for tissue engineering applications © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.