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In vitro osteoblastic differentiation of human mesenchymal stem cells and human dental pulp stem cells on poly‐ L ‐lysine‐treated titanium‐6‐aluminium‐4‐vanadium
Author(s) -
Galli Daniela,
Benedetti Laura,
Bongio Matilde,
Maliardi Valentina,
Silvani Giulia,
Ceccarelli Gabriele,
Ronzoni Flavio,
Conte Silvio,
Benazzo Francesco,
Graziano Antonio,
Papaccio Gianpaolo,
Sampaolesi Maurilio,
Cusella De Angelis Maria Gabriella
Publication year - 2011
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32996
Subject(s) - mesenchymal stem cell , dental pulp stem cells , materials science , biomaterial , stem cell , focal adhesion , periodontal ligament stem cells , microbiology and biotechnology , alkaline phosphatase , biomedical engineering , biochemistry , biology , signal transduction , nanotechnology , medicine , enzyme
Three‐dimensional (3D) titanium‐6‐aluminium‐4‐vanadium (Ti6Al4V) is a widely used biomaterial for orthopedic prosthesis and dental implants; thanks to its very high‐mechanical strength and resistance to corrosion. Human mesenchymal stem cells (hMSCs) and dental pulp stem cells (hDPSCs) are responsible for bone regeneration following colonization of prosthesis or dental implants. Both hMSCs and hDPSCs have lower ability to colonize this biomaterial in comparison with tissue culture‐treated plastic. Both hMSCs and hDPSCs show lack of focal adhesion kinase (FAK) activation when grown on Ti6Al4V. This signal is restored in the presence of poly‐ L ‐lysine (poly‐ L ‐lys). Poly‐ L ‐lys has been used as part of organoapatite or together with zinc and calcium ions. Our results suggest that poly‐ L ‐lys alone induces FAK activation through β1‐INTEGRIN, because the presence of β1‐INTEGRIN blocking antibody avoided FAK autophosphorylation. Presence of poly‐ L ‐lys also increases expression of osteoblastic differentiation marker genes in hMSCs and hDPSCs grown on Ti6Al4V. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.