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Fibrin microthreads support mesenchymal stem cell growth while maintaining differentiation potential
Author(s) -
Proulx Megan K.,
Carey Shawn P.,
DiTroia Lisa M.,
Jones Craig M.,
Fakharzadeh Michael,
Guyette Jacques P.,
Clement Amanda L.,
Orr Robert G.,
Rolle Marsha W.,
Pins George D.,
Gaudette Glenn R.
Publication year - 2011
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32978
Subject(s) - mesenchymal stem cell , fibrin , microbiology and biotechnology , materials science , cell growth , tissue engineering , biomedical engineering , cell , stem cell , regenerative medicine , viability assay , immunology , biology , medicine , biochemistry
We developed a method to produce discrete fibrin microthreads, which can be seeded with human mesenchymal stem cells (hMSCs) and used as a suture to enhance the efficiency and localization of cell delivery. To assess the efficacy of fibrin microthreads to support hMSC attachment, proliferation, and survival, microthreads (100 μm diameter per microthread) were bundled together, seeded with 50,000 hMSCs for 2 h, and cultured for 5 days. Cell density on microthread bundles increased over time in culture to a maximum average density of 731 ± 101 cells/mm 2 after 5 days. A LIVE/DEAD assay confirmed that the cells were viable, and Ki‐67 staining verified hMSC proliferation. In addition, functional differentiation assays demonstrated that hMSCs cultured on microthreads retained their ability to differentiate into adipocytes and osteocytes. The results of this study demonstrate that fibrin microthreads support hMSC viability and proliferation, while maintaining their multipotency. We anticipate that these cell‐seeded fibrin microthreads will serve as a platform technology to improve localized delivery and engraftment of viable cells to damaged tissue. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.