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In vitro and in vivo biocompatibility studies of a recombinant analogue of spidroin 1 scaffolds
Author(s) -
Moisenovich M. M.,
Pustovalova O. L.,
Yu Arhipova A.,
Vasiljeva T. V.,
Sokolova O. S.,
Bogush V. G.,
Debabov V. G.,
Sevastianov V. I.,
Kirpichnikov M. P.,
Agapov I. I.
Publication year - 2011
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32968
Subject(s) - biocompatibility , tissue engineering , scaffold , materials science , biomedical engineering , in vivo , in vitro , cell culture , biomaterial , connective tissue , 3t3 cells , biophysics , chemistry , nanotechnology , biology , biochemistry , pathology , medicine , transfection , microbiology and biotechnology , genetics , metallurgy
The goal of this study was to generate porous scaffolds from the genetically engineered protein, an analogue of Nephila clavipes spidroin 1 (rS1/9) and to assess the properties of new rS1/9 scaffolds essential for bioengineering. The salt leaching technique was used to make the rS1/9 scaffolds of interconnected macroporous structure with spontaneously formed micropores. The tensile strength of scaffolds was 18 ± 5 N/cm 2 . Scaffolds were relatively stable in a phosphate buffer but degraded in oxidizing environment after 11 weeks of incubation. Applicability of the recombinant spidroin 1 as a substrate for cell culture was demonstrated by successful 3T3 cells growth on the surface of rS1/9 films (270 ± 20 cells/mm 2 vs . 97 ± 8 cells/mm 2 on the glass surface, p < 0.01). The 3T3 fibroblasts readily proliferated within the rS1/9 scaffold (from initially plated 19 ± 2 cells/mm 3 to 3800 ± 304 cells/mm 3 after 2 weeks). By this time, cells were uniformly distributed between the surface and deeper layers (27% ± 8% and 33% ± 4%, respectively; p > 0.05), whereas the initial distribution was 58% ± 7% and 11% ± 8%, respectively; p < 0.05). The rS1/9 scaffolds implanted subcutaneously into Balb/c mice were well tolerated. Over a 2‐month period, the scaffolds promoted an ingrowth of de novo formed vascularized connective tissue elements and nerve fibers. Thus, scaffolds made of the novel recombinant spidroin 1 analogue are potentially applicable in tissue engineering. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.