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Titania–hydroxyapatite nanocomposite coatings support human mesenchymal stem cells osteogenic differentiation
Author(s) -
Dimitrievska Sashka,
Bureau Martin N.,
Antoniou John,
Mwale Fackson,
Petit Alain,
Lima Rogerio S.,
Marple Basil R.
Publication year - 2011
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32964
Subject(s) - materials science , coating , nanocomposite , mesenchymal stem cell , chemical engineering , adhesion , cell adhesion , nanotechnology , composite material , engineering , biology , microbiology and biotechnology
In addition to mechanical and chemical stability, the third design goal of the ideal bone‐implant coating is the ability to support osteogenic differentiation of mesenchymal stem cells (MSCs). Plasma‐sprayed TiO 2 ‐based bone‐implant coatings exhibit excellent long‐term mechanical properties, but their applications in bone implants are limited by their bioinertness. We have successfully produced a TiO 2 nanostructured (grain size <50 nm) based coating charged with 10% wt hydroxyapatite (TiO 2 –HA) sprayed by high‐velocity oxy‐fuel. On Ti64 substrates, the novel TiO 2 –HA coating bond 153× stronger and has a cohesive strength 4× higher than HA coatings. The HA micro‐ and nano‐sized particles covering the TiO 2 –HA coating surface are chemically bound to the TiO 2 coating matrix, producing chemically stable coatings under high mechanical solicitations. In this study, we elucidated the TiO 2 –HA nanocomposite coating surface chemistry, and in vitro osteoinductive potential by culturing human MSCs (hMSCs) in basal and in osteogenic medium (hMSC‐ob). We assessed the following hMSCs and hMSC‐ob parameters over a 3‐week period: (i) proliferation; (ii) cytoskeleton organization and cell–substrate adhesion; (iii) coating–cellular interaction morphology and growth; and (iv) cellular mineralization. The TiO 2 –HA nanocomposite coatings demonstrated 3× higher hydrophilicity than HA coatings, a TiO 2 ‐nanostructured surface in addition to the chemically bound HA micron‐ and nano‐sized rod to the surface. hMSCs and hMSC‐ob demonstrated increased proliferation and osteoblastic differentiation on the nanostructured TiO 2 –HA coatings, suggesting the TiO 2 –HA coatings nanostructure surface properties induce osteogenic differentiation of hMSC and support hMSC‐ob osteogenic potential better than our current golden standard HA coating. Published 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.