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Bone marrow stromal cells cultured on poly (lactide‐ co ‐glycolide)/nano‐hydroxyapatite composites with chemical immobilization of Arg‐Gly‐Asp peptide and preliminary bone regeneration of mandibular defect thereof
Author(s) -
Huang Yanxia,
Ren Jie,
Ren Tianbin,
Gu Shuying,
Tan Qinggang,
Zhang Lihong,
Lv Kaige,
Pan Kefeng,
Jiang Xinquan
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32922
Subject(s) - plga , materials science , stromal cell , contact angle , membrane , surface modification , x ray photoelectron spectroscopy , biomedical engineering , chemical engineering , composite material , nanotechnology , chemistry , nanoparticle , biochemistry , medicine , pathology , engineering
Polyethyleneimine (PEI) was used to create active groups on the poly (lactide‐ co ‐glycolide)/nano‐hydroxyapatite (PLGA/NHA) surface and Arg‐Gly‐Asp (RGD) was grafted on the active groups and novel PLGA/NHA 2‐D membranes and 3D scaffolds modified with RGD were obtained. X‐ray photoelectron spectrum (XPS) results show that sulfur displays only on the modified surface. The RGD‐modified PLGA/NHA materials also have much lower static water contact angle and much higher water‐absorption ability, which shows that after chemical treatment, the modified materials show better hydrophilic properties. Atomic force microscope (AFM) shows that after surface modification, the surface morphology of PLGA is greatly changed. All these results indicate that RGD peptide has successfully grafted on the surface of PLGA. Rabbit bone marrow stromal cells (MSCs) were seeded in the 2D membranes and 3D scaffolds materials. The influences of the RGD on the cell attachment, growth and differentiation, and proliferation on the different materials were studied. The modified scaffolds were implanted into rabbits to observe preliminary application in regeneration of mandibular defect. The PLGA/NHA‐RGD presents better results in bone regeneration in rabbit mandibular defect. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.