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Biocompatibility evaluation of HDPE‐UHMWPE reinforced β‐TCP nanocomposites using highly purified human osteoblast cells
Author(s) -
Shokrgozar M. A.,
Farokhi M.,
Rajaei F.,
Bagheri M. H. A,
Azari SH.,
Ghasemi I.,
Mottaghitalab F.,
Azadmanesh K.,
Radfar J.
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32892
Subject(s) - osteoblast , biocompatibility , materials science , nanocomposite , high density polyethylene , alkaline phosphatase , tissue engineering , scaffold , biomedical engineering , composite material , polyethylene , in vitro , chemistry , biochemistry , medicine , metallurgy , enzyme
Biocompatibility of β‐TCP/HDPE‐UHMWPE nanocomposite as a new bone substitute material was evaluated by using highly purified human osteoblast cells. Human osteoblast cells were isolated from bone tissue and characterized by immunofluorescence Staining before and after purification using magnetic bead system. Moreover, proliferation, alkaline phosphatase production, cell attachment, calcium deposition, gene expression, and morphology of osteoblast cells on β‐TCP/HDPE‐UHMWPE nanocomposites were evaluated. The results have shown that the human osteoblast cells were successfully purified and were suitable for subsequent cell culturing process. The high proliferation rate of osteoblast cells on β‐TCP/HDPE‐UHMWPE nanocomposite confirmed the great biocompatibility of the scaffold. Expression of bone‐specific genes was taken place after the cells were incubated in composite extract solutions. Furthermore, osteoblast cells were able to mineralize the matrix next to composite samples. Scanning electron microscopy demonstrated that cells had normal morphology on the scaffold. Thus, these results indicated that the nanosized β‐TCP/HDPE‐UHMWPE blend composites could be potential scaffold, which is used in bone tissue engineering. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.