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Gene expression, glycocalyx assay, and surface properties of human endothelial cells cultured on hydrogel matrix with sulfonic moiety: Effect of elasticity of hydrogel
Author(s) -
Yang Jing Jing,
Chen Yong Mei,
Kurokawa Takayuki,
Gong Jian Ping,
Onodera Shin,
Yasuda Kazunori
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32875
Subject(s) - self healing hydrogels , glycocalyx , materials science , biophysics , cell adhesion , extracellular matrix , microbiology and biotechnology , glycosaminoglycan , elasticity (physics) , cell culture , gene expression , adhesion , biochemistry , chemistry , polymer chemistry , composite material , biology , gene , genetics
We measured the gene expression, glycocalyx content, and surface properties of human coronary artery endothelial cells (HCAECs) cultured on poly(sodium p ‐styrene sulfonate) (PNaSS) hydrogels with various levels of elasticity ranged in 3–300 kPa. We found that all HCAECs reached confluence on these hydrogels while retaining the similar expression of EC‐specific markers to that on polystyrene (PS), a widely used scaffold in cell culture in vitro . Real‐time polymerase chain reaction (PCR) and glycosaminoglycan (GAG) assay showed that the amount of EC‐specific glycocalyx secreted by HCAECs cultured on PNaSS gels was higher than that cultured on PS, and it increased with an increase of gel elasticity. Furthermore, the HCAECs cultured on PNaSS gels showed excellent property against platelet adhesion and lower surface friction than that on PS. The platelet adhesion and surface friction of HCAECs cultured on PNaSS gels also depend on the elasticity of gels. The largest amount of EC‐specific glycocalyx, excellent blood compatibility, and the lowest friction were observed when the elastic modulus of the gel was larger than 60 kPa. Overall, HCAECs cultured on these hydrogels have better properties than those cultured on PS scaffold, demonstrating the PNaSS gels can be used as potential tissue engineering material for blood vessels. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.