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Beneficial effects of granulocyte‐colony stimulating factor on small‐diameter heparin immobilized decellularized vascular graft
Author(s) -
Zhou Min,
Liu Zhao,
Li Kun,
Qiao Wei,
Jiang Xuefeng,
Ran Feng,
Qiao Tong,
Liu Changjian
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32864
Subject(s) - decellularization , progenitor cell , neointima , granulocyte colony stimulating factor , neointimal hyperplasia , medicine , heparin , granulocyte , urology , stem cell , tissue engineering , biomedical engineering , restenosis , biology , stent , chemotherapy , genetics
Autologous recellularization of decellularized scaffolds is a promising challenge in the field of tissue‐engineered vascular graft and could be boosted by endothelial progenitor cells (EPCs). The purpose of this study was to examine the effects of granulocyte‐colony stimulating factor (G‐CSF) treatment on this process. Heparin immobilized decellularized grafts were fabricated and implanted into 48 rats, of which 25 rats received G‐CSF (50 ug/kg/day) for 14 days after operation (G‐CSF group) and other 23 received saline serving as control. Five animals of each group were euthanized at 2 weeks for analysis of early graft endothelialization; whereas the rest were investigated by Doppler ultrasound to monitor the graft patency rate up to 6 months. After 14 days of G‐CSF administration, the number of CD 34 + /CD 133 +progenitor cells was increased by 16.2 folds, and endothelial cell‐specific immunostaining revealed an enhancement of early endothelialization in G‐CSF group. After 6 months of implantation, the G‐CSF treated grafts exhibited a significantly smaller hyperplastic neointima area compared with the controls, not only at midportion (0.38 ± 0.02 vs. 0.47 ± 0.07 mm 2 , p < 0.0001), but also at distal anastomosis (0.42 ± 0.04 vs. 0.70 ± 0.13 mm 2 , p < 0.0001). Moreover, G‐CSF treated grafts had a higher patency rate compared with the control animals (19/20 vs. 12/18, p = 0.005). In conclusion, G‐CSF‐induced mobilization of circulating EPCs regenerated endothelium and inhibited neointimal hyperplasia of small‐diameter heparinized decellularized vascular graft. This cytokine therapy may be a feasible strategy for the improvement of patency rate of the novel allogeneic graft. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.