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In vitro studies of interaction of modified silica nanoparticles with different types of immunocompetent cells
Author(s) -
Kulikova Galina A.,
Parfenyuk Elena V.,
Ryabinina Irina V.,
Antsiferova Yuliya S.,
Sotnikova Nataliya Yu.,
Posiseeva Lubov V.,
Eliseeva Mariya A.
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32855
Subject(s) - materials science , nanoparticle , fluorescence , mesoporous silica , adsorption , in vitro , fluorescein isothiocyanate , amine gas treating , flow cytometry , absorption (acoustics) , biophysics , chemical engineering , nanotechnology , chemistry , microbiology and biotechnology , organic chemistry , biochemistry , composite material , biology , catalysis , mesoporous material , physics , quantum mechanics , engineering
Interactions between different types of immune cells and organically‐modified silica nanoparticles were studied. The silica particles functionalized with amine groups were prepared by sol–gel technique. Sheep immunoglobulin labeled with fluoresceine isothiocyanate was immobilized by adsorption onto the nanoparticles. The presence of the functional groups was confirmed by infrared absorption measurements. The level of immunocompetent cells interacting with the silica nanoparticles was estimated as the amount of fluorescence‐bright cells by flow cytometry method. A low level of interaction of the peripheral blood lymphocytes with the silica nanoparticles was found. On the contrary, the macrophages are actively involved in interaction with the silica nanoparticles. The influence of different size of the silica nanoparticles and incubation time on viability and functional activity of peripheral blood lymphocytes and peritoneal macrophages were investigated. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.