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In vivo evaluation of 13‐93 bioactive glass scaffolds with trabecular and oriented microstructures in a subcutaneous rat implantation model
Author(s) -
Fu Qiang,
Rahaman Mohamed N.,
Bal B. Sonny,
Kuroki Keiichi,
Brown Roger F.
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32827
Subject(s) - materials science , biomedical engineering , bioactive glass , mesenchymal stem cell , microstructure , tissue engineering , scaffold , porosity , in vivo , bone tissue , composite material , pathology , medicine , biology , microbiology and biotechnology
The ability of two groups of 13‐93 bioactive glass scaffolds to support tissue ingrowth was evaluated after implantation for 4 weeks into subcutaneous pockets in the dorsum of Fisher 344 rats. One group of scaffolds (porosity = 85%; pore size = 100–500 μm) had a “trabecular” microstructure similar to that of dry human trabecular bone, whereas the other group had a “columnar” microstructure of oriented pores (porosity = 65%; pore width = 90–110 μm). Despite the lower porosity and pore width, the columnar scaffolds supported abundant soft tissue ingrowth (glycosaminoglycan and fibrillar stroma), whereas the trabecular scaffolds showed only limited tissue ingrowth. When seeded with mesenchymal stem cells (MSCs), both groups of scaffolds supported abundant tissue infiltration. Bone‐like tissue was formed in both groups of scaffolds seeded with MSCs, but not in the scaffolds without MSCs. The new tissues integrated with the hydroxyapatite‐like surface layer of the scaffolds which resulted from the conversion of the bioactive glass in the body fluids. The results indicate that the trabecular bioactive glass scaffolds seeded with MSCs, as well as the columnar bioactive glass scaffolds, seeded with MSCs or unseeded, could serve as substrates for bone repair and regeneration. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.