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Novel poly(ethylene‐ co ‐acrylic acid) nanofibrous biomaterials for peptide synthesis and biomedical applications
Author(s) -
Xiang Bei,
Sun Gang,
Lam Kit S.,
Xiao Kai
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32819
Subject(s) - materials science , nanofiber , acrylic acid , ligand (biochemistry) , polymer chemistry , surface modification , solvent , peptide , chemical engineering , copolymer , polymer , nanotechnology , organic chemistry , biochemistry , chemistry , composite material , receptor , engineering
Poly(ethylene‐ co ‐acrylic acid) (PE‐ co ‐AA) fibers in sizes of 200–500 nm were prepared by using a novel melt‐extrusion‐extraction fabrication process. The thermoplastic nanofibers could be controllably dispersed and reassembled by a novel solvent exchange filtration method. The dispersed PE‐ co ‐AA nanofibers possess active surface areas and could directly conduct chemical reactions on surfaces. Surface modifications and organic synthesis on the nanofibers were proven effective and controllable after the dispersion. Multistep synthesis of biomolecules, such as peptide ligand HWRGWV against Fc portion of human IgG, was successful. The surface‐anchored ligand has shown bioactivity through selective binding to and staining by human IgG‐alkaline phosphatase conjugate. Another peptide, LXY3, a selective cyclic peptide ligand against α3β1 integrin of MDA‐MB‐231 breast cancer cells, was also prepared on the surfaces of the dispersed nanofibers. The results showed that MDA‐MB‐231 cells were able to specifically bind to and grow on surfaces of the nanofibers that were functionalized with LXY3. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.