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Protein adsorption on biodegradable polyanhydride microparticles
Author(s) -
CarrilloConde Brenda,
Garza Alicia,
Anderegg James,
Narasimhan Balaji
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32815
Subject(s) - ovalbumin , adsorption , bovine serum albumin , protein adsorption , materials science , sebacic acid , blood proteins , chromatography , in vivo , albumin , in vitro , chemistry , polymer chemistry , biochemistry , organic chemistry , antigen , immunology , microbiology and biotechnology , biology
The in vitro adsorption of plasma proteins on polyanhydride microparticles based on sebacic acid (SA), 1,6‐bis( p ‐carboxyphenoxy)hexane (CPH), and 1,8‐bis( p ‐carboxyphenoxy)‐3,6‐dioxaoctane (CPTEG) was studied. Three model proteins from bovine serum (albumin (BSA), immunoglobulin G (IgG), and fibrinogen (Fg)) were used. The adsorption was studied using X‐Ray Photoelectron Spectroscopy and gel electrophoresis. 2D electrophoresis was used to study the adsorption of plasma proteins from bovine serum. Differences in the amount of protein adsorbed were detected as a function of the following: (i) copolymer composition and (ii) specific protein studied. A direct correlation between polymer hydrophobicity and protein adsorbed was observed and higher quantities of Fg and IgG were absorbed. In vitro release studies were performed with ovalbumin‐encapsulated microparticles that were incubated with Fg; these studies showed a reduction in the amount of ovalbumin released from the microparticles when Fg is adsorbed on the surface. An understanding of protein adsorption patterns on parenteral delivery devices is valuable in optimizing their in vivo performance. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010