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Synthesis, in vitro macrophage response and detoxification of bamboo charcoal beads for purifying blood
Author(s) -
Hsieh MingFa,
Wen HsiaoWei,
Shyu ChihLiang,
Chen SzuHau,
Li WenTyng,
Wang WeiChieh,
Chen WenChi
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32792
Subject(s) - biocompatibility , materials science , adsorption , nitric oxide , bamboo charcoal , protein adsorption , proinflammatory cytokine , chitosan , biochemistry , immunology , composite material , chemistry , medicine , inflammation , layer (electronics) , organic chemistry , metallurgy
Bamboo charcoal beads (BCBs) were formed by coprecipitating bamboo charcoal particles with chitosan in alkaline solution. The amount of chitosan in the BCBs and their surface properties were measured. When 13–52 mg BCBs were exposed to RAW 264.7 macrophages, the amount of nitric oxide released and the cell viability were close to those of the blank. The amount of cytokine IL‐6 secreted by macrophages did not depend on the dose of BCBs but macrophages secreted more TNF‐α in response to higher doses of BCBs. However, the cytokine levels were relatively low, suggesting the favorable biocompatibility of BCBs. In adsorption experiments, BCBs adsorbed and released bovine serum albumin at particular concentrations, whereas BCBs adsorbed L ‐phenylalanine without a sign of release. This difference is attributed to the hydrophilicity and the pore size of the BCBs. Finally, the potential of BCBs as biocompatible adsorbents in blood detoxification is considered. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010