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A cellular implantation system using an injectable ultra‐purified alginate gel for repair of osteochondral defects in a rabbit model
Author(s) -
Igarashi Tatsuya,
Iwasaki Norimasa,
Kasahara Yasuhiko,
Minami Akio
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32762
Subject(s) - chondrogenesis , cartilage , biomedical engineering , stromal cell , in vitro , in vivo , materials science , tissue engineering , chemistry , anatomy , pathology , biology , medicine , biochemistry , microbiology and biotechnology
We developed a novel cellular implantation system using an in situ forming ultra‐purified alginate gel with quite low endotoxity. The aims of this study were to determine the superiority of chondrogenic potential of bone marrow stromal cells (BMSCs) cultured in the purified alginate gel compared with a commercial grade gel, and to assess reparative tissues treated with BMSCs implanted using the developed system into cartilage defects in rabbit knees. The effects of each alginate gel on cellular proliferation and chondrogenesis of rabbit BMSCs were determined by in vitro assessments. Using our purified alginate gel, a novel vehicle system for injecting BMSCs into osteochondral defects without periosteal patch was successfully established in this animal models. The in vitro analyses demonstrated that the purification of alginate significantly enhanced the cellular proliferation and chondrogenic differentiation of BMSCs. The in vivo assessments suggested that the implantation of BMSCs with the developed system using the purified alginate gel histologically and mechanically improved the reparative tissue of osteochondral defects. This system using the purified alginate gel shows the clinical potential for arthroscopically injectable implantation of BMSCs for the treatment of cartilaginous lesions. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010