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FGF‐2 abolishes the chondrogenic effect of combined BMP‐6 and TGF‐β in human adipose derived stem cells
Author(s) -
Hildner Florian,
Peterbauer Anja,
Wolbank Susanne,
Nürnberger Sylvia,
Marlovits Stefan,
Redl Heinz,
van Griensven Martijn,
Gabriel Christian
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32761
Subject(s) - chondrogenesis , glycosaminoglycan , bone morphogenetic protein 2 , fibroblast growth factor , microbiology and biotechnology , stem cell , aggrecan , transforming growth factor , materials science , chemistry , biology , pathology , anatomy , medicine , biochemistry , in vitro , osteoarthritis , articular cartilage , receptor , alternative medicine
In this study, we investigated the influence of transforming growth factor beta 3 (TGF‐β3), bone morphogenetic protein 6 (BMP‐6) and basic fibroblast growth factor (FGF‐2) on chondrogenesis in adipose derived stem cells (ASC). Cells were isolated from liposuction material, expanded and subjected to chondrogenic differentiation. Micromass pellets were cultured in chondrogenic medium containing 10 ng/mL TGF‐β3 which was additionally supplemented with 10 ng/mL BMP‐6, 10 ng/mL FGF‐2 or a combination of both. We quantitatively evaluated the cartilage specific gene expression after 14 days of culture. The end point measurements on day 35 included glycosaminoglycan (GAG) quantification, histological staining for chondrogenic markers, and transmission electron microscopy (TEM). In comparison to cultures induced with TGF‐β3/FGF‐2, the presence of TGF‐β3/BMP‐6 demonstrated strong induction of collagen type II, collagen type IX and aggrecan mRNA expression. This was corroborated by quantification and histological staining for GAGs and immunohistological staining for collagen II. However, when a combination of BMP‐6 and FGF‐2 in addition to TGF‐β3 was added, FGF‐2 counteracted BMP‐6, as indicated by reduced marker gene expression and weak to absent staining for GAGs. In conclusion, this study demonstrates that BMP‐6 combined with TGF‐β3 is a potent inducer of chondrogenesis in human ASC. In contrast, FGF‐2 does not contribute to differentiation, but rather suppresses the chondrogenic potential of BMP‐6. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010