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A new amido phosphonate derivative of carboxymethylcellulose with an osteogenic activity and which is capable of interacting with any Ti surface
Author(s) -
Barbucci Rolando,
Arturoni Enrico,
Panariello Giuseppe,
Di Canio Clara
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32757
Subject(s) - phosphonate , materials science , polymer , surface modification , potentiometric titration , adhesion , carbodiimide , osteoblast , titanium , chemical engineering , polymer chemistry , polyacrylic acid , nuclear chemistry , organic chemistry , composite material , chemistry , in vitro , biochemistry , ion , engineering , metallurgy
A new phosphonate derivative of carboxymethylcellulose (CMC) was recently synthesized (CMCAPh). The phosphonate polysaccharide was obtained by using a carbodiimide‐like activating agent for carboxylic groups and 2‐aminoethyl‐phosphonic acid to create an amide bond between the amine of the phosphonate agent and the carboxylic acids of CMC. The polymer was characterized by 31 P NMR, FT‐IR, and potentiometric titration. CMCAPh showed different properties from CMC and its amidated derivative polymer CMCA. The behavior in solution of CMCAPh polymer towards normal human osteoblasts (NHOst) was studied in vitro, monitoring the cell proliferation, cell differentiation, and osteogenic activity and was then compared with the amidic derivative of carboxymethylcellulose (CMCA). Furthermore, CMCAPh was used to coat titania disks with the aim of increasing the osteogenic activity of implant surfaces. The polymer film on the titania surface was characterized by AFM and TOF‐SIMS analysis. An ATR FT‐IR study was carried out to evaluate the polymer bonding mode onto the titanium surface. Osteoblast morphology was evaluated by SEM. Adhesion analysis of NHOst demonstrated a better adhesion on the titanium surface coated with CMCAPh than on the bare titanium surface. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010