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Cell culture platform with mechanical conditioning and nondamaging cellular detachment
Author(s) -
Lee Elaine L.,
von Recum Horst A.
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32754
Subject(s) - materials science , silicone , extracellular matrix , tissue engineering , carbodiimide , surface modification , biomedical engineering , biophysics , composite material , nanotechnology , chemical engineering , polymer chemistry , chemistry , biochemistry , medicine , biology , engineering
Cells implanted after injury may remodel undesirably with improper mechanical stimulation from surrounding tissue. Proper conditioning of tissue engineered constructs before implantation can lead to suitable tissue architectures, along with an extracellular matrix (ECM) environment that more closely mimics native tissue. Additionally, cell implantation without bulky polymeric scaffolding is often desirable. Previous researchers have created devices capable of applying mechanical forces to cells (e.g., stretch) but cellular removal from these devices, such as by trypsin, often results in irreversible damage. Conversely, devices are available that can detach intact cells, but these are inelastic, nonstretchable substrates. We have created a cell culture platform that allows for mechanical conditioning and then subsequent nondamaging detachment of those cells. We have modified silicone culture surfaces, to incorporate thermally responsive polymers of N ‐isopropylacrylamide (NIPAAm) to create an elastic substrate that can also change surface properties with temperature change. A copolymer of NIPAAm and 10% w/w acrylic acid (AAc) was conjugated to an amine‐bonded silicone surface through carbodiimide chemistry. Cells were able to attach to the resulting surfaces at 37°C and showed detachment by rounded morphology at 25°C. Following mechanical stretching, cells were still able to spontaneously detach from these modified silicone surfaces with temperature change. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010