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Differences in the bone differentiation properties of MC3T3‐E1 cells on polished bulk and sputter‐deposited titanium specimens
Author(s) -
Oya Kei,
Tanaka Yuta,
Moriyama Yoshihisa,
Yoshioka Yuki,
Kimura Tsuyoshi,
Tsutsumi Yusuke,
Doi Hisashi,
Nomura Naoyuki,
Noda Kazuhiko,
Kishida Akio,
Hanawa Takao
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32751
Subject(s) - titanium , materials science , sputtering , x ray photoelectron spectroscopy , scanning electron microscope , titanium oxide , sputter deposition , surface roughness , chemical engineering , metallurgy , composite material , thin film , nanotechnology , engineering
The roughness and cleanness of a titanium surface must be controlled in order to investigate the expression mechanism of hard tissue compatibility on titanium. In this study, osteogenic MC3T3‐E1 cells were cultured and differentiation‐induced on bulk and sputter‐deposited titanium specimens, and the osteogenesis were investigated. For the preparation of bulk specimens, titanium discs were mirror‐polished. On the other hand, titanium was sputter‐deposited on smooth and clean cover glasses as sputter‐deposited specimens. As a result, no significant difference was observed in the cell morphology and attached number. On the other hand, the time showing maximum activity in the alkaline phosphatase and gene expressions, which are related to bone differentiation on the bulk titanium, were superior to those on the sputter‐deposited titanium. From the surface observation of the specimens with a scanning electron microscope and a scanning probe microscope, the surface on the sputter‐deposited titanium was more uniform and cleaner than that on the bulk titanium. According to X‐ray photoelectron spectroscopy, the thickness of surface oxide film on the sputter‐deposited titanium was smaller than that on the bulk titanium. In addition, the proportions of TiO and Ti 2 O 3 in the surface oxide film on the sputter‐deposited titanium were larger than those on the bulk titanium. These differences might influence the differentiation of osteoblastic cells. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010