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Evaluation of scaffold materials for tooth tissue engineering
Author(s) -
Ohara Takayuki,
Itaya Toshimitsu,
Usami Kazutada,
Ando Yusuke,
Sakurai Hiroya,
Honda Masaki J.,
Ueda Minoru,
Kagami Hideaki
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32749
Subject(s) - tissue engineering , materials science , fibrin , scaffold , regeneration (biology) , biomedical engineering , dentin , biocompatibility , dental papilla , mesenchymal stem cell , microbiology and biotechnology , biology , odontoblast , composite material , medicine , metallurgy , immunology
Recently, the possibility of tooth tissue engineering has been reported. Although there are a number of available materials, information about scaffolds for tooth tissue engineering is still limited. To improve the manageability of tooth tissue engineering, the effect of scaffolds on in vivo tooth regeneration was evaluated. Collagen and fibrin were selected for this study based on the biocompatibility to dental papilla‐derived cells and the results were compared with those of polyglycolic acid (PGA) fiber and β‐tricalcium phosphate (β‐TCP) porous block, which are commonly used for tooth, dentin and bone tissue engineering. Isolated porcine tooth germ‐derived cells were seeded onto one of those scaffolds and transplanted to the back of nude mice. Tooth bud‐like structures were observed more frequently in collagen and fibrin gels than on PGA or β‐TCP, while the amount of hard tissue formation was less. The results showed that collagen and fibrin gel support the initial regeneration process of tooth buds possibly due to their ability to support the growth of epithelial and mesenchymal cells. On the other hand, maturation of tooth buds was difficult in fibrin and collagen gels, which may require other factors. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010