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Enhanced angiogenesis of modified porcine small intestinal submucosa with hyaluronic acid‐poly(lactide‐ co ‐glycolide) nanoparticles: From fabrication to preclinical validation
Author(s) -
Mondalek Fadee G.,
Ashley Richard A.,
Roth Christopher C.,
Kibar Yusuf,
Shakir Nabeel,
Ihnat Michael A.,
Fung KarMing,
Grady Brian P.,
Kropp Bradley P.,
Lin HsuehKung
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32748
Subject(s) - plga , hyaluronic acid , materials science , biomedical engineering , biocompatibility , angiogenesis , chorioallantoic membrane , cd31 , tissue engineering , zeta potential , nanoparticle , nanotechnology , anatomy , medicine , cancer research , metallurgy
Hyaluronic acid‐poly(de‐ co ‐glycolide) nanoparticles (HA‐PLGA NPs) were synthesized to stabilize the porous structure of porcine small intestinal submucosa (SIS), to improve surface biocompatibility and to enhance performance in tissue regeneration. HA‐PLGA NPs were characterized for size, zeta potential, surface morphology, and HA loading. Human microvascular endothelial cells responded to HA‐PLGA NPs and HA‐PLGA modified SIS (HA‐PLGA‐SIS) with elevated cell proliferation. HA‐PLGA‐SIS significantly enhanced neo‐vascularization in an in ovo chorioallantoic membrane angiogenesis model. The angiogenic capability of the newly fabricated HA‐PLGA‐SIS was tested in a canine bladder augmentation model. Urinary bladder augmentation was performed in beagle dogs following hemi‐cystectomy using HA‐PLGA‐SIS. The regenerated bladder was harvested at 10 weeks post augmentation and vascularization was evaluated using CD31 immunohistochemical staining. Bladder regenerated with HA‐PLGA‐SIS had significantly higher vascular ingrowth compared to unmodified SIS. This study shows that HA‐PLGA NPs may represent a new approach for modifying naturally derived SIS biomaterials in regenerative medicine. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010