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Macrophage and osteoblast responses to biphasic calcium phosphate microparticles
Author(s) -
Fellah Borhane Hakim,
Delorme Bruno,
Sohier Jérôme,
Magne David,
Hardouin Pierre,
Layrolle Pierre
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32663
Subject(s) - osteocalcin , osteoblast , alkaline phosphatase , proinflammatory cytokine , calcium , tumor necrosis factor alpha , secretion , runx2 , materials science , microbiology and biotechnology , osteopontin , macrophage , viability assay , in vitro , endocrinology , medicine , inflammation , chemistry , biology , biochemistry , enzyme
The aim of this work was to investigate in vitro the biological events leading to ectopic bone formation in contact with microporous biphasic calcium phosphate (BCP) ceramics. After implantation, microparticles may arise from their degradation and induce an inflammatory response involving macrophages. The secretion of pro‐inflammatory cytokines may affect the differentiation of osteoblasts. Mouse macrophage‐like (J774) and osteoblast‐like (MC3T3‐E1) cells were cultured in the presence of BCP microparticles of different sizes (<20, 40–80, or 80–200 μm). The smallest microparticles decreased the viability of both cell types as measured with LDH and methyl tetrazolium salt assays, and enhanced the secretion of pro‐inflammatory cytokines (IL‐6 and TNF‐α) by macrophages after 24 h, as revealed by ELISA. Osteoblastic cells were then cultured for 96 h in the presence of these pro‐inflammatory cytokines and their differentiation studied by RT‐PCR. MC3T3‐E1 cells cultured with TNF‐α showed a decrease in osterix, PTH receptor (PTHR1), and osteocalcin gene expression. On the contrary, IL‐6 enhanced the expression of osterix, Runx2, alkaline phosphatase, and osteocalcin compared with plastic. In conclusion, this study shows that the inflammatory response initiated by BCP microparticles may have both detrimental and beneficial effects on osteogenesis. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res 2010