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The effect of a nanothickness coating on rough titanium substrate in the osteogenic properties of human bone cells
Author(s) -
Moura Camilla C. G.,
Souza Maria A.,
Dechichi Paula,
ZanettaBarbosa Darceny,
Teixeira Cristina C.,
Coelho Paulo G.
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32661
Subject(s) - materials science , osteopontin , alkaline phosphatase , adhesion , coating , substrate (aquarium) , titanium , cell adhesion , viability assay , surface modification , in vitro , biophysics , composite material , chemical engineering , biochemistry , metallurgy , enzyme , chemistry , biology , immunology , ecology , engineering
This study evaluated the effect of a bioactive ceramic coating, in the nanothickness range, onto a moderately rough surface on the osteogenic behavior of human bone cells. The cells were harvested from the mandibular mental region and were cultured over Ti‐6Al‐4V disks of different surfaces: as‐machined (M), alumina‐blasted/acid etched (AB/AE), and alumina‐blasted/acid‐etched + 300–500 nm thickness amorphous Ca‐ and P‐based coating obtained by ion beam‐assisted deposition (Nano). The culture was then evaluated regarding cell viability, adhesion, morphology, immunolocalization of osteopontin (OPN) and alkaline phosphatase (ALP). The results showed that the surface treatment did not interfere with cell viability. At 1 day, AB/AE and Nano showed higher adhesion than the M surface ( p < 0.001). Higher adhesion was observed for the M than the Nano surface at 7 days ( p < 0.005). The percentage of cells showing intracellular labeling for OPN at day 1 was significantly higher for the Nano compared to M surface ( p < 0.03). The percentage of ALP intracellular labeling at 7 days was significantly higher for the AB/AE compared to the M surface ( p < 0.0065); no differences were detected at 14 days. Our results suggest that the presence of a thin bioactive ceramic coating on a rough substrate did not favor the events related to in vitro osteogenesis. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010