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NGF release from thermo‐responsive collagen‐polyNIPAam polymer networks supports neuronal cell growth and differentiation
Author(s) -
Doleski Susanne,
Yao Li,
Pandit Abhay,
Elvira Carlos
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32642
Subject(s) - materials science , neurite , nerve growth factor , biophysics , fourier transform infrared spectroscopy , swelling , polymer , poly(n isopropylacrylamide) , polymer chemistry , biomedical engineering , chemical engineering , copolymer , composite material , chemistry , biochemistry , in vitro , medicine , receptor , engineering , biology
Abstract The combination of thermo‐sensitive polymer and natural macromolecules such as collagen type I can produce biomimetic scaffolds with enhanced properties for clinical translation. This study describes the preparation of semi‐Interpenetrated Networks (semi‐IPNs) of crosslinked poly( N ‐isopropylacrylamide), or PNIPAam, with collagen type I. The collagen‐PNIPAam networks with varying collagen content were characterized using Fourier transform infrared spectroscopy techniques and their thermal responsiveness was evaluated by swelling experiments, differential scanning calorimetric (DSC) analysis. Rheological studies were performed at constant frequency and oscillating stress. PNIPAam and semi‐IPNs were preloaded with nerve growth factor (NGF), and the NGF releasing profiles at different temperatures were studied using Enzyme‐Linked ImmunoSorbent Assay (ELISA). The growth and differentiation of PC12 cells on NGF PNIPAam and collagen‐PNIPAam scaffolds were investigated in this study. Neurite length and neurite number of differentiated PC12 cells increased with the increase in collagen content in the scaffolds. © 2010 Wiley Periodicals Inc. J Biomed Mater Res Part A, 2010.