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Utilization of micelles formed from poly(ethylene glycol)‐ block ‐poly(ϵ‐caprolactone) block copolymers as nanocarriers to enable hydrophobic red two‐photon absorbing emitters for cells imaging
Author(s) -
Tian Yanqing,
Wu WenChung,
Chen ChingYi,
Jang SeiHum,
Zhang Meng,
Strovas Tim,
Anderson Judy,
Cookson Brad,
Li Yongzhong,
Meldrum Deirdre,
Chen WenChang,
Jen Alex K.Y.
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32607
Subject(s) - micelle , ethylene glycol , copolymer , caprolactone , nanocarriers , polymer chemistry , chemistry , materials science , polymer , organic chemistry , aqueous solution , drug delivery
A hydrophobic two‐photon absorbing (2PA) red emitter ( R ) was successfully incorporated into micelles formed from two block copolymers, poly(ε‐caprolactone)‐ block ‐poly(ethylene glycol)s, for imaging and toxicity studies. In micelles, the chromophore R exhibits a 2PA cross‐section of 400 GM (1 GM = 1 × 10 −50 cm 4 s photon −1 molecule −1 ) at 820 nm, which is among the highest values reported for red 2PA emitters. The micelles with a cationic amino moiety‐containing poly(ethylene glycol) corona showed an enhancement of cell internalization and delivered the dye into the cytoplasmic regions of the mouse macrophage RAW 264.7 cells. In comparison, the dye in micelles with neutral poly(ethylene glycol) as corona could not be delivered into the cells. Cytotoxicity of the micelle‐ R constructs was studied using a 3‐(4,5‐dimethyl thiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay. More than 90% of the cells were viable after they were stained with the dye‐containing micelles at different concentrations (dye concentrations of 2–6 μ M and polymer concentrations of 0.05–0.15 mg/mL) for 16 h. This is the first reported application of a hydrophobic 2,1,3‐benzothiadiazole‐containing 2PA red emitter delivered into the cytoplasm of cells for bioimaging and toxicity assessment. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010