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Effects of cell concentration and collagen concentration on contraction kinetics and mechanical properties in a bone marrow stromal cell‐collagen construct
Author(s) -
Chieh HsiaoFeng,
Sun Yulong,
Liao JiunnDer,
Su FongChin,
Zhao Chunfeng,
Amadio Peter C.,
An KaiNan
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32606
Subject(s) - contraction (grammar) , materials science , kinetics , stromal cell , viscoelasticity , biophysics , tissue engineering , stress relaxation , biomedical engineering , composite material , pathology , medicine , biology , creep , physics , quantum mechanics
Abstract A cell‐collagen construct is commonly used to investigate the phenomenon of wound healing and to estimate the variables for tissue engineering. The purpose of this study was to assess the effects of cell concentration and collagen concentration on the contraction kinetics and mechanical properties of bone marrow stromal cell (BMSC) seeded collagen lattices. To investigate the effects of both variables on the contraction kinetics, the construct contraction was monitored up to 13 days. Incremental stress– relaxation tests were carried out after a 2‐week incubation to obtain the stress–strain profiles, which were subsequently assessed in a quasilinear viscoelastic (QLV) model. During contraction, aligned BMSCs were observed first in the interior portion of the ring, followed by the middle portion and finally in the exterior portion. Constructs seeded with a higher initial cell concentration (higher than 1 × 10 5 cells/mL) or lower initial collagen concentration (lower than 2 mg/mL) exhibited faster contraction, higher ultimate stress, and superior elasticity and reduced relaxation behavior ( p < 0.05). The cell‐collagen model was successfully used to yield information regarding the initial cell concentration and the initial collagen concentration on contraction kinetics and mechanical behavior, which may have possible application in tissue engineering. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010