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Functional immobilization of interferon‐gamma induces neuronal differentiation of neural stem cells
Author(s) -
Leipzig Nic D.,
Xu Changchang,
Zahir Tasneem,
Shoichet Molly S.
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32573
Subject(s) - materials science , neural stem cell , stem cell , interferon gamma , neural activity , biomedical engineering , neuroscience , nanotechnology , biophysics , microbiology and biotechnology , biology , cytokine , immunology , medicine
Stem cell transplantation provides significant promise to regenerative strategies after injury in the central nervous system. Neural stem/progenitor cells (NSPCs) have been studied in terms of their regenerative capacity and their ability to differentiate into neurons when exposed to various soluble factors. In this study, interferon‐γ (IFN‐γ) was compared with brain‐derived neurotrophic factor (BDNF) and erythropoietin and was shown to be the best single growth factor for inducing neuronal differentiation from adult rat brain‐derived NSPCs. Next, IFN‐γ was surface immobilized to a methacrylamide chitosan (MAC) scaffold that was specifically designed to match the modulus of brain tissue and neuronal differentiation of NSPCs was examined in vitro by immunohistochemistry. Bioactive IFN‐γ was successfully immobilized and quantified by ELISA. Both soluble and immobilized IFN‐γ on MAC surfaces showed dose dependent neuronal differentiation with soluble saturation occurring at 100 ng/mL and the most effective immobilized IFN‐γ dose at 37.5 ng/cm 2 , where significantly more neurons resulted compared with controls including soluble IFN‐γ. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010