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A systematic SPR study of human plasma protein adsorption behavior on the controlled surface packing of self‐assembled poly(ethylene oxide) triblock copolymer surfaces
Author(s) -
Chang Yung,
Chu WanLing,
Chen WenYih,
Zheng Jie,
Liu Lingyun,
Ruaan RuohChyu,
Higuchi Akon
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32558
Subject(s) - materials science , protein adsorption , copolymer , adsorption , chemical engineering , ethylene oxide , polymer , biocompatibility , polymer chemistry , surface modification , monolayer , propylene oxide , oxide , organic chemistry , nanotechnology , composite material , chemistry , engineering , metallurgy
A well‐controlled biocompatible nonfouling surface is significant for biomedical requirements, especially for the improvement of biocompatibility. We demonstrate the low or nonbiofouling surfaces by coating hydrophobic–hydrophilic triblock copolymers of poly(ethylene oxide)‐poly(propylene oxide)‐poly(ethylene oxide) (PEO‐PPO‐PEO) on the CH 3 ‐terminated self‐assembled monolayer (SAM). Two types of copolymers are used to modify the surface, one with different PEO/PPO ratios (∼20/80, 40/60, and 80/20, w/w) but the same PPO molecular weight (∼2 k), the other with different copolymer MWs (∼9, 11, and 15 k) but the same PEO/PPO ratio (80/20, w/w). In situ surface plasmon resonance (SPR) sensor is used to evaluate polymer adsorption on the SAMs and subsequent protein adsorption on the copolymer‐treated surface. The effects of PEO‐PPO‐PEO molecular weight, PPO‐to‐PEO ratio, and ionic strength on protein adsorption from single protein solutions of fibrinogen, BSA, and complex mixed proteins are systematically investigated. A Pluronic™ F108 treated surface is highly resistant to nonspecific protein adsorption under the optimized conditions (MW of 15 k and PEO/PPO ratio of 80/20). This work demonstrates that the PEO‐PPO‐PEO polymer is able to achieve ultra low fouling surface via surface modification by controlling surface packing density of polymers (molecular weight, hydrophobic/hydrophilic ratio, and hydrophilic group coverage). © 2009 Wiley Periodicals, Inc. J Biomed Mater Res 2010