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Fabrication of silk fibroin blended P(LLA‐CL) nanofibrous scaffolds for tissue engineering
Author(s) -
Zhang Kuihua,
Wang Hongsheng,
Huang Chen,
Su Yan,
Mo Xiumei,
Ikada Yoshito
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32504
Subject(s) - fibroin , electrospinning , materials science , nanofiber , tissue engineering , polymer chemistry , ultimate tensile strength , solvent , random coil , chemical engineering , polymer , contact angle , nuclear chemistry , elongation , composite material , silk , biomedical engineering , chemistry , stereochemistry , organic chemistry , medicine , circular dichroism , engineering
Electrospinning using natural proteins and synthetic polymers offers an attractive technique for producing fibrous scaffolds with potential for tissue regeneration and repair. Nanofibrous scaffolds of silk fibroin (SF) and poly( L ‐lactic acid‐ co ‐ϵ‐caprolactone) (P(LLA‐CL)) blends were fabricated using 1,1,1,3,3,3‐hexafluoro‐2‐propanol as a solvent via electrospinning. The average nanofibrous diameter increased with increasing polymer concentration and decreasing the blend ratio of SF to P(LLA‐CL). Characterizations of XPS and 13 C NMR clarified the presence of SF on their surfaces and no obvious chemical bond reaction between SF with P(LLA‐CL) and SF in SF/P(LLA‐CL) nanofibers was present in a random coil conformation, SF conformation transformed from random coil to β‐sheet when treated with water vapor. Whereas water contact angle measurements conformed greater hydrophilicity than P(LLA‐CL). Both the tensile strength and elongation at break increased with the content increasing of P(LLA‐CL). Cell viability studies with pig iliac endothelial cells demonstrated that SF/P(LLA‐CL) blended nanofibrous scaffolds significantly promoted cell growth in comparison with P(LLA‐CL), especially when the weight ratio of SF to P(LLA‐CL) was 25:75. These results suggested that SF/P(LLA‐CL) blended nanofibrous scaffolds might be potential candidates for vascular tissue engineering. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010