Premium
Bacterial plaque retention on oral hard materials: Effect of surface roughness, surface composition, and physisorbed polycarboxylate
Author(s) -
McConnell Marla D.,
Liu Yu,
Nowak Andrew P.,
Pilch Shira,
Masters James G.,
Composto Russell J.
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32493
Subject(s) - enamel paint , materials science , surface roughness , adhesion , polymer , surface finish , composite material , coating , substrate (aquarium) , surface modification , chemical engineering , oceanography , engineering , geology
Bacterial adhesion to oral hard materials is dependent on various factors, for example, surface roughness and surface composition. In this study, bacteria retention on three oral hard substrates, hydroxyapatite (HAP), enamel, and polished enamel (p‐enamel) were investigated. The surface morphology and roughness of the three substrates were measured by scanning probe microscopy. HAP had the roughest surface, followed by enamel and polished enamel. For each individual substrate type, the roughness was shown to increase with scan size up to 50 μm × 50 μm. For HAP and enamel, roughness decreased considerably after formation of a pellicle, while addition of polymer coating to the pellicle layer reduced roughness much less in comparison. Bacterial surface coverage was measured at 30 min, 3 h, and 24 h on both native and surface‐modified substrates, which were coated with two different polycarboxylate‐based polymers, Gantrez S97 and Carbopol 940. As a result, the polymer coated surfaces had reduced bacteria coverage compared with the native surfaces over all time points and substrates measured. The reduction is the combined effect of electrostatic repulsion and sequestering of Ca 2+ ions at the surface, which plays a key role in the initial adhesion of bacteria to enamel surfaces in models of plaque formation. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res 2010