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Histological evaluation of chitosan‐based biomaterials used for the correction of critical size defects in rat's calvaria
Author(s) -
SpinNeto Rubens,
de Freitas Rubens Moreno,
Pavone Chaíne,
Cardoso Márcia Barreto,
CampanaFilho Sérgio Paulo,
Marcantonio Rosemary Adriana Chiérici,
Marcantonio Elcio
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32491
Subject(s) - calvaria , chitosan , biomaterial , materials science , biomedical engineering , chitin , tetracycline hydrochloride , biopolymer , dentistry , chemistry , nanotechnology , medicine , composite material , in vitro , biochemistry , polymer , antibiotics , tetracycline
Chitosan, a biopolymer obtained from chitin, and its derivates, such as chitosan hydrochloride, has been reported as wound healing accelerators and as possible bone substitutes for tissue engineering, and therefore these substances could be relevant in dentistry and periodontology. The purpose of this investigation was to make a histological evaluation of chitosan and chitosan hydrochloride biomaterials (gels) used in the correction of critical size bone defects made in rat's calvaria. Bone defects of 8 mm in diameter were surgically created in the calvaria of 50 Holtzman ( Rattus norvegicus ) rats and filled with blood clot (control), low molecular weight chitosan, high molecular weight chitosan, low molecular weight chitosan hydrochloride, and high molecular weight chitosan hydrochloride, numbering 10 animals, divided into two experimental periods (15 and 60 days), for each biomaterial. The histological evaluation was made based on the morphology of the new‐formed tissues in defect's region, and the results indicated that there was no statistical difference between the groups when the new bone formation in the entire defect's area were compared ( p > 0.05) and, except in the control groups, assorted degrees of inflammation could be seen. In conclusion, chitosan and chitosan hydrochloride biomaterials used in this study were not able to promote new bone formation in critical size defects made in rat's calvaria. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res 2010