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Patterned macroarray plates in comparison of bacterial adhesion inhibition of tantalum, titanium, and chromium compared with diamond‐like carbon
Author(s) -
Levon Jaakko,
Myllymaa Katja,
Kouri VesaPetteri,
Rautemaa Riina,
Kinnari Teemu,
Myllymaa Sami,
Konttinen Yrjö T.,
Lappalainen Reijo
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32486
Subject(s) - materials science , titanium , adhesion , tantalum , chromium , biomaterial , staphylococcus aureus , diamond like carbon , nanotechnology , metallurgy , composite material , thin film , bacteria , biology , genetics
Staphylococcus aureus device‐related infection is a common complication in implantology. Bacterial adhesion on implant surfaces is the initial step in the infective process. The aim was to develop a method suitable for quantitative bacterial adherence studies and to test a new diamond‐like carbon (DLC) coating against commonly used metallic biomaterials with regards to Staphylococcus aureus adhesion. Patterned silicon chips with spots of tantalum, titanium, chromium, and DLC were produced using ultraviolet lithography and physical vapor deposition. These patterned chips were used as such or glued to array plates, pretreated with serum and exposed to S. aureus (S‐15981) for 90 min, followed by acridine orange staining and fluorescence microscopy. An adhesion index showed that the ranking order of the biomaterials was titanium, tantalum, chromium, and DLC, with the DLC being clearly most resistant against colonization with S. aureus . Micropatterned surfaces are useful for quantitative comparison of bacterial adherence on different biomaterials. In the presence of serum, DLC is superior in its ability to resist adhesion and colonization by S. aureus compared with the commonly used biomaterial metals tantalum, titanium, and chromium. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010