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Patterning N‐type and S‐type neuroblastoma cells with Pluronic F108 and ECM proteins
Author(s) -
Corey Joseph M.,
Gertz Caitlyn C.,
Sutton Thomas J.,
Chen Qiaoran,
Mycek Katherine B.,
Wang BorShuen,
Martin Abbey A.,
Johnson Sara L.,
Feldman Eva L.
Publication year - 2010
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32485
Subject(s) - materials science , adhesion , cell adhesion , cell culture , substrate (aquarium) , adhesive , cell growth , poloxamer , nanotechnology , biophysics , polymer , chemistry , biochemistry , composite material , biology , ecology , genetics , layer (electronics) , copolymer
Influencing cell shape using micropatterned substrates affects cell behaviors, such as proliferation and apoptosis. Cell shape may also affect these behaviors in human neuroblastoma (NBL) cancer, but to date, no substrate design has effectively patterned multiple clinically important human NBL lines. In this study, we investigated whether Pluronic F108 was an effective antiadhesive coating for human NBL cells and whether it would localize three NBL lines to adhesive regions of tissue culture plastic or collagen I on substrate patterns. The adhesion and patterning of an S‐type line, SH‐EP, and two N‐type lines, SH‐SY5Y and IMR‐32, were tested. In adhesion assays, F108 deterred NBL adhesion equally as well as two antiadhesive organofunctional silanes and far better than bovine serum albumin. Patterned stripes of F108 restricted all three human NBL lines to adhesive stripes of tissue culture plastic. We then investigated four schemes of applying collagen and F108 to different regions of a substrate. Contact with collagen obliterates the ability of F108 to deter NBL adhesion, limiting how both materials can be applied to substrates to produce high fidelity NBL patterning. This patterned substrate design should facilitate investigations of the role of cell shape in NBL cell behavior. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010