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Measuring the strength of dermal fibroblast attachment to functionalized titanium alloys in vitro
Author(s) -
Pendegrass Catherine J.,
Middleton Claire A.,
Gordon David,
Jacob Josh,
Blunn Gordon W.
Publication year - 2009
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.32450
Subject(s) - fibronectin , fibroblast , materials science , dermal fibroblast , biomedical engineering , adhesion , titanium , titanium alloy , cell adhesion , in vitro , cell , composite material , alloy , medicine , metallurgy , chemistry , biochemistry
The success of intraosseous transcutaneous amputation prostheses (ITAP) relies on soft tissue attachment to prevent infection which leads to implant failure. Fibronectin (Fn) has been shown to enhance dermal fibroblast attachment in vitro , however measurement of cell attachment strength has been indirect; using cell area and immunolocalization of focal adhesion components. In this study, we have developed a flow apparatus to assess the biophysical strength of cell attachment to biomaterials used in ITAP. We have demonstrated that dermal fibroblast attachment strength increases significantly up to 96 h and that data from direct and indirect methods of assessing cell attachment strength have a significant positive correlation. Additionally, we have used direct and indirect assessment methods to demonstrate that dermal fibroblast attachment strength is significantly greater on fibronectin‐coated titanium alloy compared with uncoated controls at 1, 4, and 24 hours. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010